La identificación de criminales a través del ADN (página 8)

Partes: 1, 2, 3, 4, 5, 6, 7, 8, 9

D5S818

Other Names	Chromosomal Location	GenBank Accession
D5	5q21-q31	G08446; has 11 repeat units AC008512; has 9 repeat units

Repeat: [AGAT] = GenBank top
strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	Promega	GenePrint® PowerPlex™ 1.1
Set 2	453	5'-GGGTGATTTTCCTCTTTGGT-3' 5'-TGATTCCAATCATAGCCACA-3'
Set 3	ABI	AmpFlSTR® Profiler Plus™

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1	Set 2	Set 3	Repeat Structure	Ref.
7	119 bp	133 bp	134 bp	[AGAT]7	721
8	123 bp	137 bp	138 bp	[AGAT]8	721
9	127 bp	141 bp	142 bp	[AGAT]9	721
10	131 bp	145 bp	146 bp	[AGAT]10	721
11	135 bp	149 bp	150 bp	[AGAT]11	721
12	139 bp	153 bp	154 bp	[AGAT]12	721
13	143 bp	157 bp	158 bp	[AGAT]13	721
14	147 bp	161 bp	162 bp	[AGAT]14	721
15	151 bp	165 bp	166 bp	[AGAT]15	721
16	155 bp	169 bp	170 bp		Profiler Plus

CSF1PO

Other Names	Chromosomal Location	GenBank Accession
CSF	5q33.3-34; human c-fms proto-oncogene for CSF-1 receptor gene	X14720; has 12 repeats

Repeat: [AGAT] = GenBank top strand
(commonly used)

Reported Primers	Ref.	PCR Primer Sequences
Set 1	3	5'-AACCTGAGTCTGCCAAGGACTAGC-3' (AGAT strand) 5'-TTCCACACACCACTGGCCATCTTC-3' (TCTA strand)
Set 2	Promega	GenePrint® PowerPlex™ 1.1
Set 3	ABI	AmpFlSTR® COfiler™

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1 & Set 2	Set 3	Repeat Structure	Ref.
6	291 bp	281 bp	[AGAT]6
7	295 bp	285 bp	[AGAT]7	153
8	299 bp	289 bp	[AGAT]8	28
9	303 bp	293 bp	[AGAT]9	28
9.1	302 bp	294 bp		*variant allele*
10	307 bp	297 bp	[AGAT]10	28
10.1	308 bp	298 bp		*variant allele*
10.3	310 bp	300 bp		Budowle 1998
11	311 bp	301 bp	[AGAT]11	28
12	315 bp	305 bp	[AGAT]12	28
12.1	316 bp	306 bp		Budowle 1998
13	319 bp	309 bp	[AGAT]13	28
14	323 bp	313 bp	[AGAT]14	28
15	327 bp	317 bp	[AGAT]15	285
16	331bp	321 bp		Margolis-Nunno,H., et. al, 2001

D7S820

Other Names

Chromosomal
Location

GenBank Accession

G08616; has 12 repeat
units

AC004848; has 13 repeat
units

Repeat: [GATA] = GenBank top
strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	Promega	GenePrint® PowerPlex™ 1.1
Set 2	ABI	AmpFlSTR® Profiler Plus™
Set 3	453	5'-TGTCATAGTTTAGAACGAACTAACG-3' 5'-CTGAGGTATCAAAAACTCAGAGG-3'

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1	Set 2	Set 3	Repeat Structure	Ref.
5	211 bp	253 bp	194 bp		variant allele
6	215 bp	257 bp	198 bp	[GATA]6	721
6.3	218 bp	260 bp	201 bp		Profiler Plus
7	219 bp	261 bp	202 bp	[GATA]7	721
7.3	222 bp	264 bp	205 bp		variant allele
8	223 bp	265 bp	206 bp	[GATA]8	721
8.1	224 bp	266 bp	207 bp		variant allele
8.2	225 bp	267 bp	208 bp		variant allele
9	227 bp	269 bp	210 bp	[GATA]9	721
9.1	228 bp	270 bp	211 bp		variant allele
9.3	230 bp	272 bp	213 bp		variant allele
10	231 bp	273 bp	214 bp	[GATA]10	721
10.1	232 bp	274 bp	215 bp		variant allele
10.3	234 bp	276 bp	217 bp		variant allele
11	235 bp	277 bp	218 bp	[GATA]11	721
11.1	236 bp	278 bp	219 bp		variant allele
12	239 bp	281 bp	222 bp	[GATA]12	721
12.1	240 bp	282 bp	223 bp		variant allele
13	243 bp	285 bp	226 bp	[GATA]13	721
13.1	244 bp	286 bp	227 bp		variant allele
14	247 bp	289 bp	230 bp	[GATA]14	721
15	251 bp	293 bp	234 bp		Profiler Plus

D8S1179

Other Names	Chromosomal Location	GenBank Accession
D6S502	8q24.1-24.2	GO8710; has 12 repeat units AF216671; has 13 repeat units

Repeat: [TATC] = GenBank top strand
(called [TCTA] by FSS {375})

Primers	Ref.	PCR Primer Sequences
Set 1	CHLC Web Site, 369	5' – TTTTTGTATTTCATGTGTACATTCG – 3' 5' – CGTAGCTATAATTAGTTCATTTTCA – 3'
Set 2	PE ABI	AmpFlSTR Profiler Plus
Set 3	Promega	PowerPlex 2.1

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1	Set 2	Set 3	Repeat Structure	Ref.
7	157 bp	123 bp	203 bp	[TCTA]7	716
8	161 bp	127 bp	207 bp	[TCTA]8	369
9	165 bp	131 bp	211 bp	[TCTA]9	369
10	169 bp	135 bp	215 bp	[TCTA]10	369
11	173 bp	139 bp	219 bp	[TCTA]11	369
12	177 bp	143 bp	223 bp	[TCTA]12	369
13	181 bp	147 bp	227 bp	[TCTA]1[TCTG]1[TCTA]11	369
14	185 bp	151 bp	231 bp	[TCTA]1[TCTG]1[TCTA]12	369
15	189 bp	155 bp	235 bp	[TCTA]1[TCTG]1[TCTA]13	369
16	193 bp	159 bp	239 bp	[TCTA]2[TCTG]1[TCTA]13	369
17	197 bp	163 bp	243 bp	[TCTA]2[TCTG]2[TCTA]13	369
18	201 bp	167 bp	247 bp	[TCTA]2[TCTG]1[TCTA]15	369
19	205 bp	171 bp	251 bp	[TCTA]2[TCTG]2[TCTA]15	716

HUMTH01

Other Names	Chromosomal Location	GenBank Accession
TH01, TC11	11p15-15.5; intron 1 of human tyrosine hydroxylase gene	D00269; has 9 repeat units

Repeat: [AATG] = bottom strand
(commonly used); [TCAT] = GenBank top strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	1, 3	5'-GTGGGCTGAAAAGCTCCCGATTAT-3' (AATG strand) 5'-ATTCAAAGGGTATCTGGGCTCTGG-3' (TCAT strand)
Set 2	4	5'-GTGGGCTGAAAAGCTCCCGATTAT-3' (AATG strand) 5'-GTGATTCCCATTGGCCTGTTCCTC-3' (TCAT strand)
Set 3	125	5'-GCTTCCGAGTGCAGGTCACA-3' (AATG strand) 5'-CAGCTGCCCTAGTCAGCAC-3' (TCAT strand)
Set 4	Promega	GenePrint® PowerPlex™ 1.1
Set 5	ABI	AmpFlSTR® COfiler™
Set 6	Promega	GenePrint® PowerPlex™ 2.1

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1, 4	Set 2	Set 3	Set 5	Set 6	Repeat Structure	Ref.
3	171 bp	146 bp	230 bp	160 bp	152 bp	[AATG]3	290
4	175 bp	150 bp	234 bp	164 bp	156 bp	[AATG]4	716
5	179 bp	154 bp	238 bp	168 bp	160 bp	[AATG]5	76
5.3	182 bp	157 bp	241 bp	171 bp	163 bp		SGM Plus
6	183 bp	158 bp	242 bp	172 bp	164 bp	[AATG]6	76
6.1	184 bp	159 bp	243 bp	173 bp	165 bp		SGM Plus
6.3	186 bp	161 bp	245 bp	175 bp	167 bp	[AATG]3ATG[AATG]3	632
7	187 bp	162 bp	246 bp	176 bp	168 bp	[AATG]7	76
7.1	188 bp	163 bp	247 bp	177 bp	169 bp		SGM Plus
7.3	190 bp	165 bp	249 bp	179 bp	171 bp		SGM Plus
8	191 bp	166 bp	250 bp	180 bp	172 bp	[AATG]8	76
8.3	194 bp	169 bp	253 bp	183 bp	175 bp	[AATG]5ATG[AATG]3	76
9	195 bp	170 bp	254 bp	184 bp	176 bp	[AATG]9	76
9.3	198 bp	173 bp	257 bp	187 bp	179 bp	[AATG]6ATG[AATG]3	76
10	199 bp	174 bp	258 bp	188 bp	180 bp	[AATG]10	76
10.3	202 bp	177 bp	261 bp	191 bp	183 bp	[AATG]6ATG[AATG]4	76
11	203 bp	178 bp	262 bp	192 bp	184 bp	[AATG]11	76
12	207 bp	182 bp	266 bp	196 bp	188 bp	[AATG]12	112
13.3	214 bp	189 bp	273 bp	203 bp	195 bp	[AATG][AACT][AATG]8ATG[AATG]3	377
14	215 bp	190 bp	274 bp	204 bp	196 bp		SGM Plus

VWA

Other Names	Chromosomal Location	GenBank Accession
vWF, VWA31A	12p12-pter	M25858; has 18 (20) repeat units

Repeat: [AGAT] = bottom strand
(commonly used); [TCTA] with [TCTG] and [TCCA] inserts = GenBank
top strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	113	5'-CCCTAGTGGATAAGAATAATC-3' 5'-GGACAGATGATAAATACATAGGATGGATGG-3'
Set 1'	7	5'-CCCTAGTGGATGATAAGAATAATCAGTATG-3' 5'-GGACAGATGATAAATACATAGGATGGATGG-3'
Set 2	Promega	GenePrint® PowerPlex™ 1.1
Set 3	ABI	AmpFlSTR® Profiler Plus™

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1, 1'	Set 2	Set 3	Repeat Structure	Ref.
10	122 bp	123 bp	152 bp	TCTA TCTG TCTA [TCTG]4[TCTA]3	716
11 (13')*	126 bp	127 bp	156 bp	TCTA[TCTG]3[TCTA]7	76
12	130 bp	131 bp	160 bp	TCTA[TCTG]4[TCTA]7	7
13	134 bp	135 bp	164 bp	[TCTA]2[TCTG]4[TCTA]3TCCA[TCTA]3	716
13 (15)	134 bp	135 bp	164 bp	TCTA[TCTA]4[TCTA]8TCCATCTA	76
13 (15'')	134 bp	135 bp	164 bp	TCTA[TCTA]4[TCTA]10	76
14 (16'')	138 bp	139 bp	168 bp	TCTA[TCTG]4[TCTA]11	76
14 (16''')	138 bp	139 bp	168 bp	TCTA[TCTG]1[TCTA]1[TCTG]4[TCTA]3TCCA [TCTA]3	76
15 (17)	142 bp	143 bp	172 bp	TCTA[TCTG]4[TCTA]10TCCATCTA	76
15 (17')	142 bp	143 bp	172 bp	TCTA[TCTG]3[TCTA]11TCCATCTA	76
15.2	144 bp	145 bp	174 bp		7
16 (18)	146 bp	147 bp	176 bp	TCTA[TCTG]4[TCTA]11TCCATCTA	76
16 (18')	146 bp	147 bp	176 bp	TCTA[TCTG]3[TCTA]12TCCATCTA	76
16.1	147 bp	148 bp	177 bp		variant allele
17 (19)	150 bp	151 bp	180 bp	TCTA[TCTG]4[TCTA]12TCCATCTA	76
18 (20)	154 bp	155 bp	184 bp	TCTA[TCTG]4[TCTA]13TCCATCTA	76
18 (20')	154 bp	155 bp	184 bp	TCTA[TCTG]5[TCTA]12TCCATCTA	76
18.2	156 bp	157 bp	186 bp		SGM Plus
18.3	157 bp	158 bp	187 bp		SGM Plus
19 (21)	158 bp	159 bp	188 bp	TCTA[TCTG]4[TCTA]14TCCATCTA	76
20 (22)	162 bp	163 bp	192 bp	TCTA[TCTG]4[TCTA]15TCCATCTA	76
21 (23)	166 bp	167 bp	196 bp	TCTA[TCTG]4[TCTA]16TCCATCTA	76
22 (24)	170 bp	171 bp	200 bp	TCTA[TCTG]4[TCTA]17TCCATCTA	76
23	174 bp	175 bp	204 bp		SGM Plus
24	178 bp	179 bp	208 bp		SGM Plus
25	182 bp	181 bp	212 bp		SGM Plus

*Allele designations shown in paratheses come from
reference 76.

D13S317

Other Names	Chromosomal Location	GenBank Accession
D13	13q22-q31	G09017; has 13 repeat units AL353628.2

Repeat: [GATA] = bottom strand
(commonly used); [TATC] = GenBank top strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	Promega	GenePrint® PowerPlex™ 1.1
Set 2	453	5'-ACAGAAGTCTGGGATGTGGA-3' 5'-GCCCAAAAAGACAGACAGAA-3'
Set 3	ABI	AmpFlSTR® Profiler Plus™

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1,2	Set 3	Repeat Structure	Ref.
5	157	193		Profiler Plus
7	165 bp	201	[TATC]7	721
7.1	166	202		*variant allele*
8	169 bp	205	[TATC]8	721
8.1	170	206		*variant allele*
9	173 bp	209	[TATC]9	721
10	177 bp	213	[TATC]10	721
10'	177	213	[TATC]10 AATC	721
11	181 bp	217	[TATC]11	721
12	185 bp	221	[TATC]12	721
13	189 bp	225	[TATC]13	721
14	193 bp	229	[TATC]14	721
15	197 bp	233	[TATC]15	721
16	201	237		*variant allele*

Partes: 1, 2, 3, 4, 5, 6, 7, 8, 9

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